Sensors and Actuators B: Chemical, 2018, 142, 91-96
Wang Rui, Kim Kihyun , Choi Namhyun , Wang Xiaokun , Lee Jiyoung, Jeon Jun Ho, Rhie Gi-eun , Choo Jaebum
Abstract
Bacterial pathogens such as Yersinia pestis, Francisella tularensis, and Bacillus anthracisare classified into the highest rank of potential bioterrorism agents. Colorimetric lateral flow assay (LFA) strips are commercially available but these conventional strips have drawbacks in terms of low sensitivity and limit of . Therefore, there is an urgent need for a new sensing platform to detect these pathogens in the early contamination stage. In this study, a novel scattering (SERS)-based LFA strip was developed for sensitive detection of bacterial pathogens. Target-specific SERS nanotags (Raman reporter-labeled gold nanoparticles) were used as an alternative to the in conventional LFA strips. Using these SERS nanotags the presence of bacteria could be identified through a simple color change in the test line. Additionally, highly sensitive and accurate quantitative analysis could be performed by monitoring the characteristic intensity of SERS nanotags that were captured in the test line. This highly sensitive method required a short assay time (15?min) and a tiny volume of pathogen sample (40?μL). We believe that the proposed SERS-based LFA technique has great potential as a valuable tool in the early detection of specific bacterial pathogens in the field due to its excellent analytical sensitivity.
